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The first is the isoelectric point, abbreviated pI, which is the pH at which a peptide carries no net electrical charge

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A melting curve analysis was performed from 60 C to 85 C, and products were run on 2% agar gels to test product specificity using GelRed loading buffer and a pre-stained DNA marker (GenScript, Piscataway, NJ)

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external injuries like scrapes or foreign objects (for example a thorn in your finger)

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