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Samples were reduced with DTT (5 L of 200 mM stock in H 2 O, 10 mM final concentration) and incubated at 65 C for 15 min, then alkylated with iodoacetamide (5 L of 400 mM stock in H 2 O, 20 mM final concentration) and shaken at 37 C for 30 min in the dark

Plays a vital role in detoxification in the liver and other organs

2D and Supplementary Data S1)

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Annu Rev Biochem 1983, 52:711760

Unlike native amylin, which has an extremely short half-life of minutes, cagrilintide has been engineered for once-weekly subcutaneous administration through strategic amino acid substitutions and fatty acid acylation