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(2024) 10:e004701

The glutathionylated/oxidized enzymes were again purified via Ni-NTA affinitiy chromatograpghy followed by desalting on a PD-10 column as described above

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(5) These increases were associated with a marked decrease in oxidative stress and improvements in immune function, as indicated by a better oxidized (GSSG) to reduced (GSH) glutathione ratio and increased natural killer cell activity