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2.3 Cell culture and transfection of cells HEK-293 cells were cultured in Dulbeccos modified Eagles media, supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 g/mL streptomycin

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Genetics plays a role here, and people who have genetically lower IgE levels are less likely to have chronic urticaria.[ref] Mast cells, atrial fibrillation, and cardiac diseases: Researchers estimate that there are ~50,000 mast cells/g of human heart tissue