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Is there community support

Park EY, Shimura N, Konishi T, Sauchi Y, Wada S, Aoi W, et al

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Sands SA, Edwards BA, Terrill PI, et al

In a typical experiment, beads were washed twice with 0.5 ml of wash buffer 1 (2% SDS, all washing steps with 0.5 ml of buffer, 5 min, at room temperature), once with wash buffer 2 (0.1% w/v deoxycholate, 1% w/v Tween 20, 350 mM NaCl, 1 mM EDTA, pH 8.0), once with wash buffer 3 (0.5% w/v deoxycholate, 0.5% w/v Tween 20, 1 mM EDTA, 250 mM LiCl, 10 mM Tris-HCl, pH 7.4), once with wash buffer 4 (50 mM NaCl, 50 mM Tris-HCl, pH 7.4) and three times with 50 mM ammonium bicarbonate