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The activity of IGF-1 is in part regulated by its binding proteins
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A permease-oxidase complex involved in high-affinity iron uptake in yeast
Then we discard the culture medium and add 100 l CCK-8 solution (diluted with ECM to 10% concentration) into each well, and incubated the 96-well plates for 2 h, and the absorbance of the cells at 450 nm was measured by the multifunctional reader FlexStation 3 (Molecular Devices, CA, USA)
PLoS ONE 8(7):69161
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