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doi: 10.1038/ncomms9785 19 ChenY.HarapanahalliA

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Gonads were dissected as for germline antibody staining and fixed in 3% paraformaldehyde/0.1M K 2 HPO 4 (pH 7.2) solution for 10-20 min, followed by -20C methanol fixation for 10 min

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