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Finally, the non-adherent cells were centrifuged again, and the cell pellet was resuspended in 10 mL of culture medium (Hams F-12 medium containing 1x GlutaMAX, 15 mM HEPES, 0.8 mM CaCl, 0.25% BSA, Insulin-transferrin-selenium (ITS, Sigma, I3146), 2% FBS, and 1% P/S)

Measuring X-chromosome inactivation skew for X-linked diseases with adaptive nanopore sequencing

Welsh, W

Treatment of human fibroblasts with GHK recapitulated TGF-induced gene-expression patterns, led to the organization of the actin cytoskeleton, and elevated the expression of integrin 1

Longev., 2016, doi: 10.1155/2016/8213071