doi: 10.1016/j.ebiom.2023.104892 63 WuJ.QianY.ChenC.FengF.PanL.YangL.et al
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Preparation of NEM-derivatized cysteine and GSH internal standards The N-ethylmaleimide (NEM) derivatized isotope labeled [ 13 C 3 , 15 N]-cysteine-NEM and [ 13 C 2 , 15 N]-GSH-NEM were prepared by derivatizing the [ 13 C 3 , 15N]-cysteine and [ 13 C 2 , 15 N]-GSH standards with 50 mM NEM in 10 mM ammonium formate (pH = 7.0) at room temperature (30 min) as previously described 95
Featured Articles Frequently Asked Questions About Weight Management Over the past 30 years, the prevalence of obesity in the United States has risen60% of adults are currently obese or overweight