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If this problem persists (in spite of reducing the cell concentration), it might be alleviated by adjusting the sample to 2.5% (w/v) iodixanol, so that the cells and, in particular the debris, sediment to the first interface more slowly

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doi: 10.1016/j.jsbmb.2018.04.008 57 RosenfieldRLEhrmannDA

Porphyromonas gingivalis, a periodontal pathogen, enhances myocardial vulnerability, thereby promoting post-infarct cardiac rupture [J]

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