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High levels of oxidative stress may contribute to ALS onset, increasing motoneurons death, and heavy metals may be a cause for the increase of ROS

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A phase III clinical trial was then designed to further examine the effect of high-dose coenzyme Q 10 (1,200-2,400 mg/day) and vitamin E (1,200 IU/day) supplementation on both motor and non-motor symptoms associated with Parkinsons disease

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Cells were first gently lysed with the cytoplasmic extraction buffer to release cytoplasmic components, and the supernatant was collected after centrifugation as the cytoplasmic protein fraction