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For adenovirus infection, PDX cells or NHAs were infected with indicated adenoviruses at a multiplicity of infection (MOI) of 30 and replaced with fresh DMEM 6 h after viral infection

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PEG-precipitated virus was then re-suspended in 50 mM HEPES pH 8.0, 200 mM NaCl, 0.5% NP40 buffer, and particles pelleted on a 30% sucrose cushion