For transfection, MCF-7:2A cells were seeded in complete medium without antibiotics the day before the experiment in 12-well plates at a density of 70,000 cells per well
Glutathione acts as a major antioxidant, helping to neutralize reactive oxygen species that are generated as natural byproducts of cellular metabolism
Finally, because selection might take place at other X-linked loci, heterozygotes exhibiting extreme phenotypes by analysis of G6PD may arise from selection acting on an allele at another locus (a hitchhiking effect), as has been suggested in a family with G6PD Ilesha
For transcriptomic data analysis, BlueBee, DESeq2 R extension and IPA software were utilised for statistical analysis
If access to these peptides expands, products intended for research-use only should not be marketed for human self-experimentation
Pharmacogenomics J 2:297308 Raftogianis RB, Wood TC, Otterness DM, Van Loon JA, Weinshilboum RM (1997) Phenol sulfotransferase pharmacogenetics in humans: association of commonSULT1A1alleles with TS PST phenotype
