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Blood Collection and Biochemical Analysis At the end of the treatment period (21 days), blood samples (7ml) was obtained from the retro-orbital sinus of each animal under mild ether anaesthesia into a heparinised EDTA tubes to prevent coagulation
in addition, higher circulating IGF-1 has been linked with increases in MPS, muscle free fatty acid utilization, and improvements insulin sensitivity (Kraemer et al., 2017), which may explain its often linked importance in exercise training adaptations
The myocardium was graded for the severity of necrosis exhibited in the ventricles
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