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MuSC Proliferation In aged skeletal muscle, NNMT overexpression is linked to impaired NAD+ salvage, dysregulated SIRT1 activity, and increased muscle stem cell (MuSC) senescence

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2.5 l of protein at 1.7 mg/ml was applied to the grid thrice followed by blotting for 5 s at 100% humidity, 4C and vitrified by plunging into ethane cooled by liquid nitrogen using vitrobot