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The pellet was resuspended in 500 L lysis buffer 2 (10 mmol/L Tris, pH 8.0, 200 mmol/L NaCl, 1 mmol/L EDTA, and 0.5 mmol/L EGTA with 0.1 mmol/L PMSF and the EDTA-free protease inhibitor cocktail) and incubated at room temperature for 10 minutes

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