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Immunohistochemistry (hair cell viability)Fixed and dissected cochleae are incubated in PBS-T with 10% normal goat serum with a phalloidin primary antibody overnight at 4 C

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Historical Background and Scientific Context Scientific interest in metabolic regulatory enzymes expanded rapidly during the late twentieth century as molecular biology techniques improved

Nature 1962

These side effects typically improve as the body adjusts over time

The combination use of glutamine metabolism inhibitor and immune checkpoint inhibitor needs to be rationally designed (Yu et al., 2023)