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After blocking with 5% milk in TBST buffer, the membrane was incubated with primary antibodies followed by appropriate horseradish peroxidase-conjugated secondary antibodies (listed in Supplementary Table 1)

Defining these features may help provide a greater understanding of erythropoiesis and reveal molecular targets to regulate this process pharmacologically

Constantin-Teodosiu D, Kirby DP, Short AH, et al

117 Additionally, considered as a key antioxidative enzyme, NNT is critical for inducing macrophage inflammatory responses 118 and preventing ROS-induced cytotoxicity in T cells exposed to asbestos that can cause a reduction in antitumor immunity