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Nitric oxide response is not affected by the cystine diet Since diet composition can affect the inducible nitric oxide (NO) production capacity in response to microbial stimuli, the effect of dietary cystine was investigated in an LPS experiment

Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol

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You are checking for clarity, meaning you should be able to see through the liquid clearly