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Collagen IV (Col IV), laminin, and nuclei were visualized after staining with anti-Col IV (green), anti-LanB1 (red), and DAPI (blue), respectively

From a clinical perspective, large-scale, longitudinal studies are needed to evaluate the robustness of GBB and L-CN as early biomarkers across diverse populations, considering inter-individual variability in microbiota composition, diet, and genetic background

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