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After verifying oxidizing influences on Mia40 in vivo , we next wanted to test which reducing pathways act on Mia40 or more specifically whether the redox state of Mia40 is susceptible to changes in E GSH (Figure 8A)

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On that basis a 1 mL draw delivers about 125 mg combined MIC and ~1000 mcg B12, and a 0.5 mL draw delivers half of each

Nuclear magnetic resonance spectroscopy 420 l of culture medium, 120 l of 1 mM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS) in deuterium oxide (D 2 O) as an internal standard, and 60 l of 500 mM phosphate buffer, pH 7 were mixed and transferred to 5 mm glass NMR tubes for analysis

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