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For serum preparation, blood samples were incubated for 1 h at room temperature and then centrifuged (1,950 x g, 15 min, Varifuge 3.0, Heraeus, Hanau, Germany) and stored at -80C prior to further analysis

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How Tirzepatide Weight Loss Works Appetite Suppression : Tirzepatide helps curb hunger by influencing hormone signals that regulate appetite

By stimulating both GLP-1 and GIP receptors, it helps reduce appetite and slow digestion, keeping you feeling full longer