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It works by blocking an enzyme called NNMT (nicotinamide N-methyltransferase)

That is an important framing for anyone tempted to treat it as a shortcut: even in the animal work that generates the interest, the compound is one input alongside energy balance, not a replacement for it

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These studies often employ techniques like ELISA or Western blotting to measure protein expression and qPCR to analyze gene transcription levels