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Cells were washed once with 1 ml PBS then lysed in the plate with 0.1 ml/well 2X Laemmli buffer (Bio-Rad) supplemented with 20 mM DTT

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This is why intestinal integrity is best understood as a regulated system involving epithelial cells, tight junctions, mucus protection, immune signaling, and microbial balance

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Several studies and our mesh meta-analysis suggest that TZDs improve IR and dyslipidaemia in PCOS more than metformin does [163, 214, 215]