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By kinetic analysis of single, double, and triple mutants, as well as from studies of reaction products, it was established that the enzyme A216H catalyzes the hydrolysis of the thiol-ester substrate by a mechanism that includes an acyl intermediate at the side chain of Y9

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In addition, ATF4 overexpression reduced the inhibition of HuOCSC proliferation caused by the ferroptosis agonist erastin (Figure 5A), whereas siATF4 has the opposite effect (Figure 6A)

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