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A Bradford Protein Assay Kit (Bio BASIC INC., Ontario, Canada, Cat# SK3041) was then used to fully extract the proteins

Store upright at controlled room temperature 15C to 30C (59F to 86F)

Reduced Sunlight: In the winter, people often spend less time outdoors, leading to decreased exposure to sunlight

Gene knockout was performed using the one-step inactivation method of Datsenko and Wanner [32]

Compounded Medication Disclosure: Compounded semaglutide and compounded tirzepatide available through Eden's pharmacy network are not FDA-approved medications