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The serum samples were kept at -20C pending biochemical determinations which included total testosterone, free testosterone, 8-hydroxy-2-deoxyguanosine (8-OHdG) and advanced glycation end products (AGEs) that were measured by enzyme linked immunosorbent (ELIZA) using ELIZA kits (Sunlong, China, Catalogue Number SL1061Ra, SL0293Ra, SL0019Ra, SL0036Ra respectively) according to the operatings instructions

doi: 10.1385/BTER:112:2:119 110 KnezevicJStarchlCTmava BerishaAAmreinK

Some individuals may need a higher number of sessions to achieve their desired results

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The key is controlled, methodical observation