Prebiotics delivered to the air cell are transferred through the vascular system in the chorioallantoic membrane into the intestine, while probiotics in the air cell are available for the chicken embryo at the time of hatch ( in ovo delivery, including vitamins, amino acids, carbohydrates, prebiotics, probiotics, synbiotics, hormones, minerals, CpG oligodeoxynucleotides, proteins, and immunostimulants (Figure 1)
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