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Increasing metabolic activity not only triggers the production of free oxygen radicals but also disrupts the antioxidant defense mechanism

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Quantification of GSH using Ellmans assay Cells were harvested and lysed with 0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5 to afford protein free lysate