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All other analytical-grade chemicals and reagents, including Griess reagent, phosphate-buffered saline (PBS), TRIzol reagent, normal saline, absolute ethanol, xylene, hematoxylin, and eosin, were supplied by the Department of Biomedical Sciences, PAF Institute of Applied Sciences and Technology (PAF-IAST), Pakistan

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11 Conversely, other researchers have used the AOM model and observed no changes in immunoblot tight junction protein expression

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