This complete, ready-to-use kit includes clear 96-well plate(s), glutathione standard, assay buffer, and other components to perform the assay
Hsieh, M.-J., Liu, H.-T., Wang, C.-N., Huang, H.-Y., Lin, Y., Ko, Y.-S., Wang, J.-S., Chang, V
With BPC-157: No interaction
In another set of experiments, ASA treatments (5 or 10 mol/ml ASA 48 h) were performed in GSH-depleted cells (BSO treatment for 18 h) which were then treated with 10 mM NAC (dissolved in DMSO, then diluted with cell culture medium to the required concentration keeping the DMSO concentration not more than 0.1%) for 2 hr before the addition of ASA for 48 h to manipulate cellular GSH pools
So, when we say glutathione is reduced, we mean it can remove oxygen or ROS hence its being an "antioxidant" after which the glutathione becomes oxidized
[5] After completion of this reaction, glutathione reductase recycles oxidized glutathione back to the reduced form so that it again can be picked up by GSTs