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Controlled drug delivery vehicles for cancer treatment and their performance

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Sub-2.5 mg dosing depends on product format, clinician supervision, and current compounding rules

The fix: maintain at least 3 sites in rotation, ideally 5-6

The plasmid for the non-tagged SUN2 expression was cloned by cutting the SUN2-CFP plasmid 65 with EcoRI and BamHI, blunting with Phusion DNA Polymerase (New England Biolabs, M0530), and re-ligating the plasmid thus introducing a stop codon in frame with the SUN2 sequence