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The standard GHK-Cu injection frequency is once daily, five days on and two days off, for 4 to 12 weeks followed by a 2 to 4 week break

Montagne A, Barnes SR, Sweeney MD, et al

You may feel brief pressure, warmth or mild discomfort at the injection site

Li, S., Wang, L., Zhang, X., Chai, H., Huang, Y.: A Co, N co-doped hierarchically porous carbon hybrid as a highly efficient oxidase mimetic for glutathione detection

And there is a couple of reasons for that, but one of them is that oxidants such as hydrogen peroxide are preventing the fatty acids from getting into the mitochondria and they are all being left over