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Undigested cross walls and debris were removed by centrifugation (10 min at 50,000 g ) and crude nozzle preparations were collected in the ultracentrifuge (1 h at 366,000 g ) before being further purified using a CsCl density gradient (0.3 g/ml)

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Repeated freeze-thaw cycles promote peptide degradation

Henan Chuangxin Biological Technology Co., Ltd

The multivariate triple cross-product added 55 more compounds with 39 elevated in males