If you're taking high-dose D3, periodic lab monitoring of 25-OH D and calcium is the responsible approach especially during a period of active weight loss when your body composition and nutrient dynamics are changing
Once peptide denaturation or chemical degradation has occurred, returning the vial to the refrigerator does not undo the damage
PLoS ONE 8 , e76520 (2013)
If you consistently have significant volume remaining at the 28-day mark, reduce your reconstitution volume or purchase smaller vials matched to your actual usage rate
For the assessment of body composition, the bioelectrical impedance analysis method was employed utilising a sophisticated body composition device, namely the InBody S10 (BioSpace)
Preparation of DAB-stained HEK293T cells for EM LaccID-expressing cells were fixed using warm 2% glutaraldehyde in buffer (100 mM sodium cacodylate with 2 mM CaCl 2 , pH 7.4) and then quickly moved to ice