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If the issues and deficiencies cant be addressed through an interactive review, then FDA will send the manufacturer an Additional Information request

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The powdered tissue was lysed in RIPA lysis buffer (Servicebio, G2002) supplemented with 1% phosphatase inhibitor (Epizyme, GRF102) and 1% protease inhibitor (Epizyme, GRF101), followed by agitation at 4 for 30 min