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The remaining H 2 O 2 was removed by desalting spin columns according to the manufacturer's manual (Zeba Spin Desalting Columns, www.thermoscientific.com)

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100 nuclei from more than 20 animals were examined for each group ( b )

Eight days of swimming exercise significantly increases the expression of the decay-accelerating factor 16 (DAF-16) in the L4-stage hidradenitis elegans nematodes, followed by PINK1 mRNA expression, which prolongs the lifespan of hidradenitis elegans nematodes by promoting autophagy and mitophagy (Mikhail et al., 2023)

The samples were centrifuged at 4700 g for 2 min at 25C