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Residues of the agonist are indicated by the chain (P) denotation and GLP-1R residues are indicated by the (R) denotation

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On the day of the experiment, the culture medium was replaced with DMEM containing 5.6 mM glucose and 0.1% bovine serum albumin (BSA) and the cells were then serum starved for 3 hours while equilibrated in a tissue culture incubator

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