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found that treatment with disulfiram (DSF) increased the production of cytoplasmic and mitochondrial ROS in MM cells, led to the loss of mitochondrial membrane potential and an increase in the level of lipid peroxidation, and caused a significant downregulation of ferroptosis - related genes including GPX4 ( T-5224 is an activator protein-1 (AP-1) inhibitor that specifically blocks the DNA-binding activity of the c-Fos/c-Jun heterodimer, thereby suppressing the expression of downstream pro-inflammatory cytokines such as TNF- and matrix metalloproteinases like MMP-3

Author Nigel Barton References 1a

Deliberately Monochrome: Hes always depicted in black and white, a nod to the film he originates in

Subsequently, the cells were washed with PBS and blocked for 1 h with 1% bovine serum albumin (BSA, Sigma A9418) and 22.52 mg/mL glycine in PBST (PBS + 0.05% Tween-20, LPS Solution CBP007T)