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Lactate Dehydrogenase (LDH) Assay for Cytotoxicity Cytotoxicity was determined using an LDH Assay Kit (Beyotime, China), in accordance with the manufacturers instructions

Endothelial cell tube formation assay To examine the effect of BPC-157 on angiogenesis in vitro, tube formation assay was performed as described previously.28 In this assay, we used two study protocols