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After incubation of 10 h, B16 cells were dyed with SYTOX green (10 nM) for 10 min at room temperature and imaged by fluorescence microscope (Leica, DMI6000B, Germany)

The frequency at which each enzyme was listed as a tier-1 target is provided in Supplementary Data 8

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Swelling or hyperalgesia may reflect peritendinous irritation

Remarkably, significant conformational changes of ECL1 upon receptor activation were observed relative to their inactive conformations: (i) in the inactive GCGR, ECL1 forms a -hairpin conformation and further compacts -sheet with the stalk region