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We have previously demonstrated that low picomolar concentrations of PregS are sufficient to increase [Ca 2+ ]i and CREB phosphorylation (374)

& Kang, S

However, when performing a similar pH titration with the U597C variant minimal changes in the formation of the thiolate-FAD charge transfer are observed (Figure 9A) suggesting both that the 571 histidine residue is the acting general base and the C159 residue is highly shielded from solvent

In 2023, Puja Das et al

So understanding mechanisms of tolerance is a great challenge and may help to find new strategies to tackle this side effect

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