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A two-step polymerase chain reaction (PCR) workflow was conducted for library preparation in accordance with procedures described in the Illumina 16S sample preparation guide

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Class 2: Alogliptin and linagliptin are targeted at specific areas: S1, S2, and sometimes S1` and S2 ` , Comparatively, linagliptin is almost eight times stronger than alogliptin [7], [8], [17], [20]