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Similarly, the expression of key autophagy genes, such as ATG5 , ATG7 , and BECN1 , is downregulated during normal aging in the human brain [209]

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Histological examination with HE and PAS staining showed glomerular cell proliferation, enlargement of the mesangial matrix, and increase in the thickness of the basement membrane, confirming the proper establishment of the DKD model (Figure 3A)