Then we discard the culture medium and add 100 l CCK-8 solution (diluted with ECM to 10% concentration) into each well, and incubated the 96-well plates for 2 h, and the absorbance of the cells at 450 nm was measured by the multifunctional reader FlexStation 3 (Molecular Devices, CA, USA)
Zambrano S, Blanca AJ, Ruiz-Armenta MV, Miguel-Carrasco JL, Revilla E, Santa-Mara C, Mate A, Vzquez CM
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Similar to caSMCs, we found that GLP-1(2836) increased (mitochondrial) glucose oxidation in caECs (145.4 2.7 vs