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These gastrointestinal effects are usually mild to moderate and often improve as your body adjusts to the medication

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The samples were then sonicated and centrifuged at 10,000 g for 10 min

Similarly, double-heterozygous-mutant worms carrying both the D72G and G247Vfs*6 alleles (modeling the two patients carrying the compound heterozygous variants) showed no embryonic lethality, even under prior starvation (Fig