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GSSG was higher and GSH:GSSG lower in asthmatic compared with HC BAL fluid, while intracellular GSH was lower in asthma
After mixing the sample or standard with ThioStar and incubating at room temperature for 15 minutes, the GSH-generated signal is read at 510 nm in a fluorescent plate reader with excitation at 390 nm
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Bottom line: They answer different questions
While most people dont think twice about it, some may notice an odd side effect afterwards a metallic taste in the mouth
Furthermore, the systemic neuroinflammation driven by this oxidative stress directly impacts the autonomic nervous system
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