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In a previous study, we found that a high microbial diversity was associated with reduced MAIT cell activation in vitro ( Staphylococcus aureus cell-free supernatant ( To get a comprehensive overview of the behavior of different immune populations after stimulation with chemically treated SIHUMIx, we performed t-SNE analysis which allows to identify rare or even unknown populations and to compare the expression patterns of cell and activation markers between different experimental groups ( + MAIT cells and DN MAIT cells

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Garcia-Fernandez M, Sierra I, Puche JE, Guerra L, Castilla-Cortazar I

Samples were diluted with ammonium bicarbonate (AMBIC) 50 mM pH 7.8 and trypsin was added in a 1:20 enzyme: protein ratio for overnight digestion at 37 C

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