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The spectral changes at both excitation wavelengths followed identical kinetics, which allowed us to reliably monitor the formation of roGFP2 WT (S 2 ), roGFP2 C151S (SSG), or roGFP2 C208S (SSG) using PfGrx C32S/C88S (SSG) as an oxidant (Fig

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Give each site a minimum of 7 days to recover before using it again