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Primary antibodies including Ki67 (1:200, Abcam, London, UK), Cleaved caspase-3 (1:1000, CST, Danvers, MA), -catenin (1:200, CST, Danvers, MA), IGF-1 (1:250, Abcam, London, UK), AR (1:250, Abcam, London, UK), K14 (1:100, Proteintech, Chicago, USA) were incubated overnight at 4

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